Frequently Asked Questions
1) Can the device be used for the transport or storage of the corneoscleral button?
No, the Re-One is designed specifically for the entire eyeball.
2) What transport liquid should be used in the Re-One?
The doctor performing the removal decides which transport liquid is to be used. We recommend a liquid composed of Gentamicin sulphate and glucose 5%. During transport, the eyeball must be completely immersed in the transport liquid.
3) How much liquid can be contained in the device?
The device can hold approximately 35 ml of liquid (without eyeball).
4) How long can you leave the eyeball in the Re-One?
The eyeball must arrive at destination as fast as possible. The length of stay in the device of the eyeball should not exceed 48 hours. The device is suitable only for short-term storage. After cutting the corneoscleral button, this may be stored in containers and special liquids for longer periods awaiting transplant.
5) The internal cover (in the 02 model) serves only for observation under the microscope. Do I have to carry this separately from the device containing the eyeball?
No, you can carry the internal cover assembled to the device containing the eyeball: The internal cover has no use during transport, but screwing it to the eye wrapper it can be transported to the destination with the device.
Warning: the internal cover is screwed only partially to the eye wrapper so it is not in contact with the eye bulb.
6) How do you correctly position the internal cover for the observation of the corneal endothelium under the microscope?
The internal cover must be screwed in order to make contact with the eyeball (at the corneal limbus level): doing so you create a small enclosed compartment where you can pour in the working liquid so as to keep the cornea hydrated during microscopic observation. If there is no contact between the corneal limbus and the internal cover the working liquid is not retained and will fall to the bottom.
7) Which working liquid should be used?
This is at the discretion of the medical professional. Usually liquids are utilized that have a basis of antibiotics and saline solution (For example Gentamicin sulphate and NaCl 0,9%).
8) Removal from the cadaver: What are the advantages of the removal of the eyeball whole in respect to direct collection of the corneoscleral button?
Some institutions prefer to use the technique of removal of the corneoscleral button directly from the cadaver. This procedure, however, if performed by inexperienced hands, puts at risk the integrity of the cornea and currently too many corneas are rendered useless by inappropriate handling. The withdrawal of the entire eyeball is much safer to avoid corneal damage, especially if specialists do not perform the removal.